Method of selectively determining a fungal biomass

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/04 (2006.01) C12Q 1/25 (2006.01) C12Q 1/34 (2006.01) G01N 33/569 (2006.01) G01N 33/573 (2006.01)

Patent

CA 2279378

A method for selectively determining a fungal biomass by detection of a fungal enzymatic activity that is present in substantially all fungal species, such as enzymes involved in chitin metabolism (chitinase, chitin synthase, chitosanase, N-acetyl-glucosaminidase and .beta.-N-acetylhexosamidase). The invention can be used for detecting a fungal biomass in environmental samples, food products, plant materials, building materials, industrial fungal cultures or sample from a human being or animal including blood samples. In particular, 4-methylumbelliferyl N-acetyl-.beta.-D-glucosaminide is used as substrate for .beta.-N-acetylhexosamidase (EC 3.2.1.52). This enzyme activity correlates with the amount of fungal biomass present in a sample.

Procédé de détermination sélective d'une biomasse fongique par la détection d'une activité enzymatique fongique présente dans sensiblement toutes les espèces fongiques, telles que les enzymes impliquées dans le métabolisme de la chitine (chitinase, chitine synthase, chitosanase, N-acétyl-glucosaminidase et beta -N-acétylhexosamidase). Le procédé de l'invention peut être utilisé pour la détection d'une biomasse fongique dans des échantillon prélevés dans l'environnement, dans des produits alimentaires, du matériel végétal, des matériaux de construction, des cultures fongiques industrielles ou dans un échantillon prélevé sur un humain ou un animal, dont les échantillons sanguins. Le 4-méthylombelliféryl-N-acétyl- beta -D-glucosaminide est utilisé comme substrat pour la beta -N-acétylhexosamidase (EC 3.2.1.52). Cette activité enzymatique est liée à la quantité de biomasse fongique présente dans l'échantillon.

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