Method of separating cells from a sample

Details Method of separating cells from a sample Method of separating cells from a sample

C - Chemistry, Metallurgy

12

N

C12N 5/09 (2010.01) C12N 5/071 (2010.01) A61K 35/12 (2006.01) A61K 35/14 (2006.01) B01L 3/14 (2006.01) C07K 1/22 (2006.01) C12M 3/00 (2006.01) G01N 1/28 (2006.01) G01N 33/537 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2365925

Rare cells are separated from a sample fluid by a positive selection or negative selection antibody by centrifuging in a tube containing a harvesting float. The harvesting float has an axial passage and a density to settle in the sample fluid and expand the layer of the target component. The antibody is preferably coupled to a particulate carrier, such as a microbead, to attach either the target component or a contaminating component to the particulate carrier. In the positive separation; the particulate carrier is recovered in the axial passage of the float.

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