C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
C07K 1/18 (2006.01) C07K 14/575 (2006.01) A61K 38/00 (2006.01)
Patent
CA 2160703
This invention relates to a method for separating glycosylated proteins from non-glycosylated proteins. The method comprises the following steps: 1) equilibrating the protein containing solution with an equilibrating solution containing at least one organic solvent; 2) adding the equilibrated protein solution onto a chromatographic column containing a packing for the ion-exchange affinity separation of glycosylated and non-glycosylated proteins; 3) eluting the glycosylated proteins from the chromatographic column with a sufficient quantity of a first eluting solution containing at least one organic solvent and with or without at least one salt, wherein the salt concentration and/or organic solvent concentration and/or pH are appropriate for the eluting of glycosylated proteins being substantially free of non-glycosylated proteins; and 4) eluting the non-glycosylated proteins from the chromatographic column with a sufficient quantity of a second eluting solution containing at least one organic solvent and with or without at least one salt, wherein the salt concentration and/or organic solvent concentration and/or pH are appropriate for the eluting of non-glycosylated proteins being substantially free of glycosylated proteins.
Genzyme Corporation
Smart & Biggar
LandOfFree
Method of separating glycosylated and non-glycosylated proteins does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method of separating glycosylated and non-glycosylated proteins, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of separating glycosylated and non-glycosylated proteins will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1732886