Method of sequencing

Details Method of sequencing Method of sequencing

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2117603

2117603 9320232 PCTABS00027 The present invention provides a method of sequencing two or more regions of target DNA wherein each of said regions is annealed to a different sequencing primer followed by DNA synthesis using a sequencing polymerase and sequencing nucleotides with subsequent size grading of the DNA so synthesised, each of said sequencing primers being provided with separate distinguishing and/or immobilisation means except that one of said sequencing primers may lack such means, whereby the populations of DNA synthesised from each DNA region may be distinguished and/or separated and a kit for carrying out such a method.

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