Method of sequencing a nucleic acid

Details Method of sequencing a nucleic acid Method of sequencing a nucleic acid

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) B01J 19/00 (2006.01)

Patent

CA 2384510

Disclosed herein are methods and apparatuses for sequencing a nucleic acid. In one aspect, the method includes annealing a population of circular nucleic acid molecules to a plurality of anchor primers linked to a solid support, and amplifying those members of the population of circular nucleic acid molecules which anneal to the target nucleic acid, and then sequencing the amplified molecules by detecting the presence of a sequence by-product such as pyrophosphate.

L'invention concerne des méthodes et des appareils de séquençage d'un acide nucléique. Selon un aspect, le procédé consiste à anneler une population de molécules circulaires d'acide nucléique à une pluralité d'amorces d'ancrage liées à un support solide; à amplifier les membres de la population de molécules circulaires d'acide nucléique s'annelant à l'acide nucléique cible, puis à séquencer les molécules amplifiées par détection de la présence d'un sous-produit de séquence tel que le pyrophosphate.

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