Method of solubilizing, purifying, and refolding protein

Details Method of solubilizing, purifying, and refolding protein Method of solubilizing, purifying, and refolding protein

C - Chemistry, Metallurgy

07

K

C07K 14/81 (2006.01) A61K 9/08 (2006.01) A61K 38/57 (2006.01) A61K 47/30 (2006.01) A61K 47/36 (2006.01) C07K 1/113 (2006.01) C07K 1/14 (2006.01)

Patent

CA 2223745

A method of modifying protein solubility employs polyionic polymers. These facilitate the solubilization, formulation, purification and refolding of proteins especially incorrectly folded proteins and aggregated proteins. Compositions are described that are suitable for formulating TFPI. The compositions allow preparation of pharmaceutically acceptable compositions of TFPI at concentrations above 0.2 mg/mL and above 10 mg/mL.

La présente invention se rapporte à un procédé pour modifier la solubilité de protéines utilisant des polymères polyioniques. Lesdits polymères facilitent la solubilisation, la formulation, la purification et le repliement de protéines, particulièrement pour les protéines incorrectement pliées et les protéines en agrégats. L'invention concerne des compositions qui sont adéquates pour formuler l'inhibiteur des voies du facteur tissulaire (TFPI). Les compositions permettent la préparation de compositions de TFPI pharmaceutiquement acceptables à des concentrations supérieures à 0,2 mg/ml et supérieures à 10 mg/ml.

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