Method of stabilizing enzyme conjugates

Details Method of stabilizing enzyme conjugates Method of stabilizing enzyme conjugates

C - Chemistry, Metallurgy

12

N

C12N 11/02 (2006.01) C12N 9/96 (2006.01) G01N 33/535 (2006.01) G01N 33/542 (2006.01) G01N 33/573 (2006.01)

Patent

CA 2055812

ABSTRACT OF THE DISCLOSURE A method is disclosed for stabilizing a conjugate of an enzyme and a member of a specific binding pair (enzyme conjugate). The method comprises the step of combining the enzyme conjugate with an effective amount of an antibody for the enzyme where the antibody does not substantially inhibit the activity of the enzyme. The invention has application to assays for the determination of an analyte wherein enzyme conjugates are employed. The improvement comprises employing as a reagent in the assay an immune complex of an enzyme conjugate and an antibody for the enzyme where the antibody does not substantially inhibit the activity of the enzyme. Compositions comprising such an immune complex and kits comprising such an immune complex in packaged combination with other assay reagents are also disclosed. 3380.01 27810-FF

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