Method of stabilizing reagent for amplifying or detecting...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/09 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2450397

A method of stabilizing a reaction reagent for highly sensitively and specifically amplifying a target nucleic acid in a sample with the use of a chimeric oligonucleotide primer and a method of storing the same over a long time; and a method of highly sensitively detecting a pathogenic microorganism and a virus.

L'invention concerne un procédé de stabilisation d'un réactif pour amplifier avec sensibilité et de manière spécifique un acide nucléique cible dans un échantillon à l'aide d'une amorce d'oligonucléotide chimérique. L'invention traite aussi d'un procédé de stockage de ce dernier sur une longue période. Enfin, l'invention a aussi pour objet un procédé permettant de détecter avec une haute sensibilité un micro-organisme pathogène et un virus.

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