Method of stabilizing recombinant hepatitis b virus surface...

Details Method of stabilizing recombinant hepatitis b virus surface... Method of stabilizing recombinant hepatitis b virus surface...

C - Chemistry, Metallurgy

07

K

530/3.12, 530/3.

C07K 14/02 (2006.01) C07K 1/36 (2006.01) C07K 14/01 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2031354

Recombinant hepatitis B virus surface proteins produced in yeast are rapidly and efficiently purified from yeast cell extracts in a high pH buffer. The yeast cell extract is heat treated, cooled and the pH is reduced. The surface proteins are then adsorbed onto wide pore silica followed by elution and concentration. This method eliminates the requisite introduction of protease inhibitors, stabilizes the surface protein and improves product yield.

Des protéines de surface d'un virus recombinant de l'hépatite B obtenu dans des levures sont rapidement et efficacement séparées d'extraits cellulaires et purifiées dans un milieu tamponné à pH élevé. Les extraits cellulaires de levures sont traités thermiquement et refroidis, et le pH est abaissé. Ensuite, les protéines de surface sont adsorbées sur de la silice à porosité élevée, soumises à un traitement par élution et concentrées. Cette méthode permet de se passer de l'introduction d'inhibiteurs de la protéase, stabilise les protéines de surface et accroît le rendement.

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