Method of staining micro-organisms

Details Method of staining micro-organisms Method of staining micro-organisms

C - Chemistry, Metallurgy

12

Q

150/16.4

C12Q 1/24 (2006.01) C12Q 1/04 (2006.01) G01N 1/30 (2006.01)

Patent

CA 1089339

ABSTRACT OF THE DISCLOSURE A rapid method of staining micro-organisms with fluorochrome dyes to render them readily visible when illuminated with U.V. radiation and viewed under the micro- scope is described. The method involves the chemical treat- ment of the sample containing micro-organisms with phosphate ions to modify chemically the dye-receptor sites in the micro- organisms. Suitable additional treatments which enhance fluor- escence are also possible, including methylation, esterification, hydrolysis, oxidation and treatment with sulphur dioxide. The method is particularly advantageous in the microscopic enumer- ation of micro-organisms. Also certain techniques are described which permit the determination of viable and non-viable cells. After the cells have been chemically treated with phosphate ion they are stained with a fluorochrome dye.

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