Method of stirring solution

Details Method of stirring solution Method of stirring solution

G - Physics

01

N

G01N 35/02 (2006.01) C12M 1/02 (2006.01) C12N 15/09 (2006.01) C12Q 1/68 (2006.01) G01N 1/36 (2006.01) G01N 33/53 (2006.01) G01N 37/00 (2006.01)

Patent

CA 2559768

A method of agitating a solution, comprising providing a substance capable of selective binding immobilized on a carrier surface, bringing a solution containing a test substance capable of reacting with the selective binding substance into contact with the selective binding substance and performing agitation of the solution, wherein the test substance containing solution is mixed with fine particles or air bubbles, the fine particles or air bubbles moved without contact with the selective binding substance immobilization surface. This solution agitating method realizes acceleration of the reaction between the selective binding substance immobilized on the carrier and the test substance and even when the amount of analyte is minute, realizes providing of an agitation method excelling in signal intensity and S/N ratio. This solution agitating method contributes to realization of diagnosis and inspection at clinical sites utilizing a carrier having a selective binding substance immobilized thereon, such as DNA chip.

Procédé d~agitation d~une solution, comprenant la préparation d~une substance capable d~une liaison sélective immobilisée sur une surface de support, la mise au contact d~une solution contenant une substance témoin capable de réagir avec la substance de liaison sélective avec la substance de liaison sélective et l~agitation de la solution, dans lequel la solution contenant une substance témoin est mélangée à des particules fines ou à des bulles d~air, les particules fines ou les bulles d~air se déplaçant sans venir au contact de la surface d~immobilisation de la substance de liaison sélective. Ce procédé d~agitation d~une solution permet d~accélérer la réaction entre la substance de liaison sélective sur le support et la substance témoin, même lorsque la quantité d~analyte est minuscule, permet de proposer un procédé d~agitation offrant une intensité de signal et un rapport signal/bruit excellents. Ce procédé d~agitation d~une solution contribue à réaliser un diagnostic et une inspection au niveau de sites cliniques à l~aide d~un support sur lequel est immobilisée une substance de liaison sélective, comme une puce d~ADN.

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