Method of storing and/or transporting in vitro cell cultures

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 5/00 (2006.01)

Patent

CA 2530318

The invention relates to a method of storing and/or transporting in vitro two- dimensional cell cultures. The inventive method comprises the following steps consisting in: a) coating a cell culture that is immobilised on an asymmetric support with a gelatine solution in culture medium at a concentration of between 1 and 5 %; b) solidifying the gelatine added to the support at a temperature of between 15 and 25 ~C; and c) storing and/or transporting the cell culture at a temperature of between 15 and 25 ~C for a period of up to 96 hours. The invention also relates to a kit which is used to store and/or transport the in vitro two-dimensional cell cultures according to the inventive method, said kit comprising: i) an asymmetric support, and (ii) a gelatine solution in culture medium at a concentration of between 1 and 5 %.

L'invention concerne une méthode destinée au stockage et/ou au transport de cultures cellulaires bidimensionnelles organisées in vitro, consistant : a) à recouvrir d'une solution de gélatine en milieu de culture à une concentration de 1-5 % une culture cellulaire organisée immobilisée sur un support asymétrique, b) à solidifier à une température de 15-25 ·C la gélatine ajoutée au support et c) à stocker et/ou à transporter la culture cellulaire à une température de 15-25 ·C durant une période pouvant aller jusqu'à 96 heures. La présente invention concerne également une trousse destinée au stockage et/au transport de cultures cellulaires bidimensionnelles organisées in vitro selon la méthode décrite ci-dessus, comprenant i) un support asymétrique et ii) une solution de gélatine en milieu de culture à une concentration de 1-5 %.

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