Method of synthesising phytosphingosine-containing ceramides...

C - Chemistry – Metallurgy – 12 – P

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C12P 13/00 (2006.01) A61K 8/68 (2006.01) C07C 69/675 (2006.01) C07C 231/02 (2006.01) C07C 235/08 (2006.01) C07C 235/28 (2006.01) C07D 303/42 (2006.01) C07H 15/04 (2006.01) C07H 15/10 (2006.01) A61K 7/06 (1995.01) A61K 7/40 (1995.01) A61K 7/48 (1995.01)

Patent

CA 2148139

2148139 9410131 PCTABS00032 An efficient method of producing phytosphingosine-containing ceramide one having general structure (1), comprising: (a) obtaining a phytosphingosine base from tetraacetylphytosphingosine (TAPS) by a deacetylation reaction wherein the TAPS is produced by fermentation of cells of the F-60-10 mating type strain of Hansenula ciferrii) using a fed-batch mode and a non-fermentable carbon source; and (b) coupling together the phytosphingosine base and a fatty acid/.omega.-hydroxy fatty acid component wherein the .omega.-hydroxy fatty acid component is prepared by a process which includes Kolbé synthesis.

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