Method of synthesizing oligonucleotides adaptable for large...

Details Method of synthesizing oligonucleotides adaptable for large... Method of synthesizing oligonucleotides adaptable for large...

C - Chemistry, Metallurgy

07

H

C07H 21/00 (2006.01) B01J 19/00 (2006.01)

Patent

CA 2051692

2051692 9012024 PCTABS00002 A method of producing synthetic oligonucleotides on a small or large scale using H-phosphonate nucleoside monomers is described. The method can be used to synthesize oligonucleotides of any length, including oligodeoxyribonucleotides and oligoribonucleotides. The method results in a coupling efficiency of greater than 97 % and consumes only two to three equivalents of monomer to activator per coupling reaction. In addition, the method does not require a separate capping step and capping reagent because the activating reagent serves a self-capping function thereby preventing elongation of failed sequences. The H-phosphonate linkages of the fully synthesized oligonucleotide can be oxidized with a variety of reagents to obtain either phosphate diester or other types of modified oligonucleotides.

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