Method of tacrolimus extraction and quantification using...

G - Physics – 01 – N

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G01N 33/50 (2006.01) C07D 498/18 (2006.01)

Patent

CA 2625649

The present invention relates to a method of extracting tacrolimus in a test blood sample to use in a tacrolimus quantification assay. The method of the present invention extracts tacrolimus in non-precipitating, non-denaturing aqueous environment, avoiding cumbersome manual pretreatment procedures or the use of organic solvents. The method of the present invention permits full automation of tacrolimus quantification assays.

La présente invention porte sur un procédé d'extraction de tacrolimus dans un échantillon sanguin de test destin é à être utilisé dans un essai de quantification. Ce procédé permet d'extraire tacrolimus dans un environnement aqueux non précipité, non dénaturant, ce qui évite des procédures de prétraitement manuelles lourdes ou l'utilisation de solvants organiques. Le procédé de cette invention permet une totale automatisation des essais de quantification de tacrolimus.

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