Method of targeted gene disruption, genome of...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/31 (2006.01) C07H 21/02 (2006.01) C07K 14/195 (2006.01) C12N 15/11 (2006.01) C12N 15/74 (2006.01) C12N 15/90 (2006.01) C12Q 1/68 (2006.01) C40B 40/06 (2006.01) C40B 40/08 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2497338

It is intended to provide an efficient and sure gene targeting method embodied at an arbitrary position in the genome of an organism and a kit therefor. It is also intended to provide a method for tartgetedly disrupting an arbitrary gene in the genome of an organism which comprises: 1) the step of providing the whole sequencial data of the genome of the organism; 2) the step of selecting at least one arbitrary region in the sequence; 3) the step of providing a vector containing a sequence homologous with the region selected above and a marker gene; 4) the step of transforming the organism by the vector; and 5) the step of providing the organism under such conditions as allowing homologous recombination. Moreover, the genome of a hyperthermostable bacterium and its array are provided.

La présente invention vise à fournir un procédé efficace et fiable pour le ciblage de gènes inclus à une position arbitraire dans le génome d'un organisme et une trousse pour sa mise en oeuvre. L'invention vise également à fournir un procédé pour la disruption ciblée d'un gène arbitraire dans le génome d'un organisme comprenant les étape suivantes: 1) la fourniture de la donnée séquentielle complète du génome de l'organisme ; 2) la sélection d'au moins une région arbitraire dans la séquence ; 3) la fourniture d'un vecteur contenant une séquence homologue avec ladite région sélectionnée et un gène marqueur ; 4) la transformation de l'organisme par le vecteur ; et 5) la fourniture de l'organisme dans des conditions permettant une recombinaison homologue. L'invention a également trait au génome d'une bactérie hyperthermostable et son jeu ordonné d'échantillons.

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