Method of testing drug for treating or preventing diseases...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/09 (2006.01) C07K 14/47 (2006.01) C07K 16/08 (2006.01) C12N 1/15 (2006.01) C12N 1/19 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 15/12 (2006.01) C12P 21/02 (2006.01) C12P 21/08 (2006.01) C12Q 1/02 (2006.01) C12Q 1/68 (2006.01) G01N 33/15 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2450022

It is intended to provide a novel method of testing a remedy or a preventive for at least one disease selected from among hyperlipemia, arteriosclerosis and hyperglycemia. More specifically, a method which comprises culturing cultured cells in the presence or absence of a test substance, detecting the mRNA expression dose, promoter activity or protein production level of ~angiopoetin-associated protein 3 or 4~ gene, and thus selecting a test substance showing a lowered mRNA expression dose, promoter activity or protein production level thus detected in the cells cultured in the presence thereof compared with the cells cultured in the absence thereof.

L'invention concerne un nouveau procédé destiné à tester un remède ou un agent prophylactique contre au moins une maladie choisie parmi l'hyperlipémie, l'artériosclérose et l'hyperglycémie. Plus particulièrement, l'invention concerne un procédé qui consiste à cultiver des cellules de culture en présence ou en absence d'une substance de test, à détecter la dose d'expression d'ARNm, l'activité du promoteur ou le niveau de production de protéines d'un gène "d'une protéine 3 ou 4" associé à l'angiopoïétine, puis à sélectionner une substance de test présentant une dose d'expression d'ARNm abaissée, l'activité du promoteur ou le niveau de production de protéines ainsi détecté dans les cellules cultivées en présence de cette substance est comparé à celle cultivée en l'absence de celle-ci.

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