A - Human Necessities – 61 – L
Patent
A - Human Necessities
61
L
167/327, 195/34.
A61L 27/00 (2006.01) A61L 27/34 (2006.01) A61L 27/38 (2006.01) A61L 27/50 (2006.01)
Patent
CA 1293700
A METHOD OF TREATING A SYNTHETIC OR NATURALLY OCCURRING SURFACE WITH A COLLAGEN LAMINATE TO SUPPORT MICROVASCULAR ENDOTHELIAL CELL GROWTH, AND THE SURFACE ITSELF Abstract A novel implantable prosthesis for implantation in human patients is disclosed which comprises a synthetic substrate and a Type IV/V collagen surface layer seeded with a confluent monolayer of autologous endothelial cells. In the preferred embodiment, a base layer of interstitial collagen is adhered to the substrate. The Type IV/V collagen surface layer is provided in a laminate comprising an intermediate layer of interstitial collagen which is covalently bound to the aforementioned base layer. A cross linking agent, such as glutaraldehyde, is utilized to covalently bind the interstitial collagen base layer to the substrate, and the collagen intermediate layer to the base layer. The cross linking agent is subsequently deactivated with a soluble peptide, such as lysine. Autologous endothelial cells, such as microvascular endothelial cells derived from adipose tissue, are then seeded at high densities on the pretreated graft material. Those cells adhere in suitable percentages within times compatible with most vascular surgical procedures, and, even more importantly, flatten on that surface to assume the appearance of a natural, cobblestone morphology. The subject surface is resistant to shear stress, and yields good results when tested as a vena cava implant in dog.
510929
Jarrell Bruce Evans
Williams Stuart Konradd
Kirby Eades Gale Baker
Thomas Jefferson University
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