C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/70 (2006.01)
Patent
CA 2081354
The present invention relates to a method of the type- specific detection of herpes simplex virus, characterized by comprising the step of DNA amplification in a liquid mixture containing either of a combination of a first DNA primer containing an oligonucleotide having at least 15 bases in a base sequence of the formula (1a) CACGGGTATA AGGACATCCA (1a) with a second DNA primer containing an oligonucleotide having at least 15 bases in a base sequence of the formula (2a): GGGTCCTCGT CCAGATCGCT (2a), or a combination of a first DNA primer containing an oligonucleotide having at least 15 bases in a base sequence of the formula (1b): GCCTCTTTTC CCCCGGGGAG (1b) with a second DNA primer containing an oligonucleotide having at least 15 bases in a base sequence of the formula (2b): GGGAAAAAAG CCGCGCCGGGG (2b), a DNA polymerase and an aqueous liquid sample; and then the step of DNA examination on the resultant reaction solution. According to the present invention, it is possible to differentiate infectious disease caused by the HSV I or HSV II, accurately and rapidly in a type specific manner.
Kita Hiroshi
Kurimura Takashi
Matsumoto Toshiya
Iatron Laboratories Inc.
Mcfadden Fincham
Mitsubishi Chemical Medience Corporation
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