Method of unsupervised cell nuclei segmentation

G - Physics – 06 – T

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G06T 5/00 (2006.01) G06T 7/60 (2006.01)

Patent

CA 2333614

A method of segmentation of cell nuclei is described that uses an active contours approach based on a Viterbi search algorithm. Also disclosed is an initialisation technique that maximises the probability of a correct segmentation. The invention also includes a method to classify cell nuclei according to the difficulty of correct segmentation. Nuclei that are difficult to segment can be rejected to minimise the probability of false positives. The probability of false positives can be driven close to 0%.

L'invention porte sur un procédé de segmentation de noyaux cellulaires qui utilise une méthode des contours actifs sur la base d'un algorithme de recherche Viterbi. L'invention porte également sur une technique d'initialisation qui augmente la probabilité d'une segmentation correcte. Cette invention porte en outre sur un procédé visant à classifier des noyaux cellulaires selon la difficulté de segmentation correcte. Les noyaux difficiles à segmenter peuvent être rejetés de façon à réduire la probabilité de faux positifs, probabilité pouvant atteindre presque 0 %.

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