Method of using antibodies against human luteinizing...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 5/00 (2006.01) C07K 16/26 (2006.01) G01N 33/50 (2006.01) G01N 33/531 (2006.01) G01N 33/569 (2006.01) G01N 33/574 (2006.01) G01N 33/577 (2006.01) G01N 33/76 (2006.01)

Patent

CA 2132867

The invention provides a method for detecting the presence of human malignant cells in a sample of tumor cells. which comprises contacting the sample with an antibody directed to an epitope on the beta subunit of human luteinizing hormone or on intact human luteinizing hormone under conditions such that the antibody forms a complex with cells in the sample if the epitope is present on the surface of the cells. The invention also provides a method for determining whether a tumor present in a human subject is malignant, which comprises obtaining a sample of cells from the tumor and detecting the presence of malignant cells in the sample according to the method of the invention. The invention provides a method for obtaining an enriched population of live human malignant cells which comprises contacting a population of cells comprising human malignant cells with an antibody directed to an epitope on the beta subunit of human luteinizing hormone or on intact luteinizing hormone under conditions such that the antibody forms a complex with the cells present in the population, and isolating the cells which form a complex with the antibody.

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