Method of using dna episomes to suppress gene expression in...

Details Method of using dna episomes to suppress gene expression in... Method of using dna episomes to suppress gene expression in...

C - Chemistry, Metallurgy

12

N

C12N 15/82 (2006.01) C12N 15/83 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2410492

The introduction of DNA episomes into plant cells to reduce or prevent the expression of endogenous plant genes is described. Geminivirus vectors to provide silencing, preferably systemic silencing, of endogenous plant genes in a plant are described. Further provided are methods of silencing one or more plant genes, for example, to reduce unwanted gene products or for rapid screening of gene function in plants.

L'introduction d'épisomes d'ADN dans des cellules végétales permet de réduire ou prévenir l'expression des gènes endogènes dans les plantes. Des vecteurs de géminivirus peuvent entraîner la mise en veille, de préférence la mise en veille systémique, des gènes endogènes dans les plantes. L'invention porte également sur des procédés de mise en veille d'un ou plusieurs gènes de végétaux, par exemple, pour réduire des produits géniques non désirés ou pour rechercher rapidement la fonction génique des plantes.

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