Method of using preserved control cells in the calibration...

Details Method of using preserved control cells in the calibration... Method of using preserved control cells in the calibration...

G - Physics

01

N

G01N 37/00 (2006.01) C12N 1/20 (2006.01) C12N 5/00 (2006.01) G01N 15/10 (2006.01) G01N 21/64 (2006.01) G01N 21/77 (2006.01) G01N 33/50 (2006.01) G01N 33/533 (2006.01) G01N 33/554 (2006.01) G01N 33/58 (2006.01) G01N 15/14 (2006.01)

Patent

CA 2153344

The invention describes a method of using differently labelled, reconstituted preserved cells as control cells in multiple color assays. These cells are used to calibrate light scatter and fluorescent intensity measurements. The use of said control cells overcomes the preservation and calibration difficulties experienced in the conventional use of fresh cells and polymer beads as controls in determining the compensation adjustments required in multiple color flow cytometry assays as a result of the overlapping wavelength regions encountered.

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