Method, reagent and kit for genotyping of human papillomavirus

Details Method, reagent and kit for genotyping of human papillomavirus Method, reagent and kit for genotyping of human papillomavirus

C - Chemistry, Metallurgy

12

Q

C12Q 1/70 (2006.01) C07H 21/04 (2006.01)

Patent

CA 2324978

The sequence of human papillomavirus present in a sample is determined by amplifying a portion of the L1 open reading frame of human papillomavirus genome to form L1 amplicons containing plus and minus amplified strands using MY09 and MY11 amplification primers, and then determining the positions of at least the A bases in the antisense amplicon using a consensus sequencing primer which is shifted six bases with respect to the MY09 primer (MY09-6). This primer has the sequence ARRGGAWACT GATCWARDTC (Seq. ID No. 3).

On détermine la séquence du papillomavirus humain présent dans un échantillon en amplifiant une partie du cadre de lecture ouvert L1 du génome de ce papillomavirus humain, afin de former des amplicons L1 contenant des brins amplifiés plus et moins utilisant des amorces d'amplification MY09 et MY11, et en déterminant ensuite les positions au moins des bases A dans l'amplicon antisens utilisant une amorce de séquençage consensus qui est décalée de 6 bases par rapport à l'amorce MY09 (MY09-6). Cette amorce comporte la séquence ARRGGAWACT GATCWARDTC (numéro d'identification de séquence 3).

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