Method reagent and kit for the detection and amplification...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01) C12N 15/10 (2006.01) C12P 19/34 (2006.01) C12Q 1/70 (2006.01)

Patent

CA 2141537

2141537 9403630 PCTABS00030 Methods and reagents for the detection and exponential amplification of target nucleic acid molecules are disclosed. The method generally employs a Primer Oligonucleotide which hybridizes in concert with a Blocker Oligonucleotide on a strand of the target molecule, and an End-Run Oligonucleotide which can hybridize to the Blocker Oligonucleotide.

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