C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
150/16.5
C12Q 1/04 (2006.01) C12Q 1/18 (2006.01) C12Q 1/24 (2006.01)
Patent
CA 1101769
AHP-6852-1-Cl 071378 - cl ABSTRACT OF THE DISCLOSURE: The disclosure is directed to a method, reagents and apparatus for the rapid (one to four hours) identification of Neisseria gonorrhoeae and Neisseria meningitidis from cultures grown on a selective or a non-selective medium. One drop of growth suspended in sterile salt solution is placed in each of a plurality of miniature reaction chambers supported on a common base and incubated at 35-37°C for one to four hours. A dried substrate and buffer is contained in each chamber. A detector reagent such as a diazo dye in an aqueous solution with a polar solvent is added to each chamber after incubation. The color change in each chamber is noted and compared with a profile to identify N. gonorrhoeae and Neisseria meningitidis. Synthetic substrates rather than sugars are used in the reaction chambers. The substrates are naphthyl derivatives, .beta.-naphthyl or .beta.-naphthylamides, and are 1) .beta.-naphthyl-.beta.,D-galactopyranoside 2) N-L-.lambda.-glutamyl-.beta.-naphthylamide 3) L-hydroxyproline-.beta.-naphthylamide 4) L-serine-.beta.-naphthylamide 5) L-arginine-.beta.-naphthylamide 6) glycine-glycine-.beta.-naphthylamide 7) .beta.-naphthyl-phosphate 8) .beta.-naphthyl-valerate 9) 4 methoxy leucine-.beta.-naphthylamide 10) glycine-.beta.-naphthylamide -24-
312691
D'amato Richard F.
Eriquez Louis A.
American Home Products Corporation
Gowling Lafleur Henderson Llp
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