Method, test article, and system for performing assays

Details Method, test article, and system for performing assays Method, test article, and system for performing assays

C - Chemistry, Metallurgy

12

Q

C12Q 1/00 (2006.01) C12M 1/40 (2006.01) C12Q 1/56 (2006.01) G01N 27/04 (2006.01) G01N 27/327 (2006.01)

Patent

CA 2152498

Assays for liquid analytes are performed on a bibulous matrix containing dried reagents which produce a visibly detectable reaction product Application of liquid sample to the bibulous matrix is detected by measuring a drop in resistance across the matrix. A preferred test article for performing the method includes the matrix and a pair of spaced-apart electrodes in electrical contact with a reaction zone on the matrix. The test article is used in combination with a detection unit having means for probing the electrodes to determine when electrical resistance in the matrix has decreased. The assay methods and apparatus are particularly useful for preforming enzyme assays where signal developed as a function of time is critical.

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