Method, test device and kit for assay of specific binding...

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G01N 33/569 (2006.01) G01N 33/538 (2006.01) G01N 33/543 (2006.01) G01N 33/547 (2006.01)

Patent

CA 2076592

METHOD, TEST DEVICE AND KIT FOR ASSAY OF SPECIFIC BINDING LIGAND USING CONTROLLED FLOW THROUGH FILTRATION MEMBRANE Abstract of the Disclosure A test device has a microporous filtration membrane and absorbing means in fluid contact therewith which are designed so as to provide a drainage time of at least about 20 seconds for a given fluid sample. Retaining the fluid on the membrane for a longer period of time during a specific binding assay reduces background and improves assay sensitivity. The test device can be supplied as part of a diagnostic test kit and used in a variety of diagnostic methods. In particular, the invention is useful for the determination of microorganisms associated with periodontal diseases.

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