Method, test element and kit for semi-quantitative detection...

Details Method, test element and kit for semi-quantitative detection... Method, test element and kit for semi-quantitative detection...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12M 1/34 (2006.01) C12Q 1/70 (2006.01)

Patent

CA 2145957

A target nucleic acid can be detected in a semi-quantitative fashion by passing it over detection deposits in a test element. The detection deposits include particles affixed to the test element, some of which particles have capture probe attached thereto, and other particles having no capture probe. The deposits have varying amounts of capture probe so that the signal obtained when the target nucleic acid is captured thereon can be semi-quantitatively correlated to the amount of target nucleic acid in the specimen. This method of detection can be used in nucleic acid hybridization assays or following amplification methods, including polymerase chain reaction.

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