Method to control gene expression in bacteria, namely...

Details Method to control gene expression in bacteria, namely... Method to control gene expression in bacteria, namely...

C - Chemistry, Metallurgy

12

N

C12N 15/74 (2006.01) A01N 63/00 (2006.01) C07K 14/195 (2006.01) C12N 15/31 (2006.01) C12N 15/70 (2006.01)

Patent

CA 2348775

A promintron sequence derived from an intervening sequence of the rolA gene of Agrobacterium rhizogenes strain A4 is described. The sequence is able to drive gene expression within bacteroids in all stages of nodule development in order to obtain, over the developmental time of the nodule, a constitutive expression of the gene(s) of interest. Uses of said sequence, derived vectors and recombinant bacteria are also described.

L'invention concerne une séquence ci-après dénommée <=promintron>=, cette séquence étant dérivée d'une séquence intermédiaire du gène ro1A de la souche d'Agrobacterium rhizogenes A4. Cette séquence est capable de réguler l'expression génique dans des bactéroïdes à chaque étape du développement du nodule, ce qui permet d'obtenir, sur toute la durée du développement de ce nodule, une expression constitutive du(des) gène(s) recherché(s). Cette invention concerne également l'utilisation de cette séquence, ainsi que des vecteurs dérivés et des bactéries recombinantes.

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