Method to detect cytotoxic effectors

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/32 (2006.01) C12Q 1/02 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2112306

METHOD TO DETECT CYTOTOXIC EFFECTORS ABSTRACT A method to detect cytotoxic effectors, comprising the steps as follows: to incubate some target cells into a solution of said effector; to separate the supernatant from the incubation mixture, to detect at least one microsomial enzyme in this supernatant by means of an enzyme reaction; to establish the ER (Experimental Release) value of said microsomial enzyme by deducting a control value from the obtained value, being the control value obtained by exposing said target cells to a solution, that is free from said cytotoxic effector.

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