Method to detect dirofilaria immitis infection

Details Method to detect dirofilaria immitis infection Method to detect dirofilaria immitis infection

C - Chemistry, Metallurgy

12

N

C12N 15/12 (2006.01) C07K 14/435 (2006.01) G01N 33/564 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2266428

The present invention includes a method to detect D. immitis infection in a host animal using a D. immitis Di33 protein to detect anti-D. immitis Di33 antibodies in a bodily fluid of the animal. Also included is a method to detect D. immitis infection in a host animal using a D. immitis anti-Di33 protein to detect Di33 proteins in a bodily fluid of the animal. The present invention also relates to D. immitis detection kits that include either a Di33 protein or an anti-Di33 antibody; such kits also include a composition to detect an immunocomplex between the anti-Di33 antibody and D. immitis Di33 protein. The present invention also includes Di33 proteins, nucleic acid molecules encoding such proteins, as well as recombinant molecules and recombinant cells comprising such nucleic acid molecules, and anti-Di33 antibodies. Also included are methods to produce such proteins, nucleic acid molecules and antibodies.

L'invention concerne une méthode permettant de détecter une infection à D. immitis chez un animal hôte, qui utilise une protéine Di33 de D. immitis pour détecter les anticorps anti-protéine Di33 de D. immitis dans un liquide corporel dudit animal. Elle concerne également un méthode permettant de détecter une infection à D. immitis chez un animal hôte, qui utilise la protéine Di33 de D. immitis pour détecter les protéines Di33 dans un liquide corporel dudit animal. Elle concerne aussi des trousses de détection de D. immitis qui comprennent soit une protéine Di33, soit un anticorps anti-Di33, ainsi qu'une composition permettant de détecter un complexe immun entre l'anticorps anti-Di33 et la protéine Di33 de D. immitis. Elle concerne également des protéines Di33, des molécules d'acides nucléiques codant pour lesdites protéines, ainsi que des molécules recombinantes et des cellules recombinantes contenant lesdites molécules d'acides nucléiques et des anticorps anti-Di33. L'invention concerne enfin des méthodes permettant de produire lesdites protéines, lesdites molécules d'acides nucléiques et lesdits anticorps.

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