C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
530/15.12, 195/1
C12N 15/70 (2006.01) C07K 14/31 (2006.01) C07K 14/65 (2006.01) C12N 1/38 (2006.01) C12N 15/62 (2006.01) C12N 15/67 (2006.01) C12N 15/74 (2006.01) C12P 21/02 (2006.01)
Patent
CA 1314010
ABSTRACT A process for expressing proteins in Gram(-) bacteria and providing for extracellular secretion thereof, comprising the steps: a) introducing into a Gram(-) bacterium a recombinant DNA construction comprising a promoter, a signal sequence enabling translocation and processing, and a structural gene encoding the desired protein to be expressed; b) cultivating the bacterium under conditions resulting in filamentous growth; and c) recovering the extracellularly secreted protein; a recombinant DNA construction comprising: a promoter, a signal sequence and a structural gene inclu- ding a cleavage region, wherein the structural gene is of the formula: (E)n(B)m - Y, where n is an integer ? 1, m is an integer, Y is a DNA sequence encoding a desired protein and E and B are the genes correspond- ing to the protein A regions E and B, with the proviso that the structural gene is different from that encoding natural protein A; a plasmid vector comprising such recombinant DNA construction; a Gram(-) bacterium harbouring said recombinant DNA construc- tion; and proteins obtained by such process.
525185
Abrahmsen Lars
Moks Tomas
Nilsson Bjorn
Uhlen Mathias
Fetherstonhaugh & Co.
Pharmacia & Upjohn Aktiebolag
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