Method to grow in vitro til cells

Details Method to grow in vitro til cells Method to grow in vitro til cells

C - Chemistry, Metallurgy

12

N

C12N 5/02 (2006.01) C12N 5/06 (2006.01) C12N 5/08 (2006.01)

Patent

CA 2115112

A METHOD TO GROW IN VITRO TIL CELLS ABSTRACT A method to grow in vitro TIL cell is described, comprising the steps as follows: - a single cell dissolution of a tumourous tissue; - a said cell first incubation in a culture medium with a serum, that allows the lymphocyte growth, comprising interleukin 2 and phytolectin, for an interval from 3 to 5 days at 37°C in order to provide a primary culture; - an isolation of mononucleated cell of said primary culture; and - a second incubation of said monucleated cells in a culture medium, that allows the lymphocytes growth, comprising Interleukin-2, for an interval from 10 to 20 days at 37°C. Preferably, the method is used to grow TIL cells from NSSC.

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