C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 9/42 (2006.01) C12N 9/24 (2006.01)
Patent
CA 2385937
The present invention relates to protein engineering, and concerns especially family G/11 xylanases, and genes encoding said enzymes. In specific, the invention concerns Trichoderma reesei XYNII gene, which codes for endo-1,4-.beta.-xylanase (EC 3.2.1.8). The invention describes how site-directed mutagenesis can be used to improve the properties of an enzyme to match the industrial conditions where it is used. Protein engineering can be used to improve thermoactivity and thermostability of xylanases, as well as to broaden their pH range.
Cette invention, qui a trait à l'ingénierie des protéines, concerne notamment des xylanases de la famille G/11 ainsi que des gènes codant ces enzymes. Elle porte, de manière spécifique, sur le gène Trichoderma reesei XYNII qui code l'endo-1,4-.beta.-xylanase (EC 3.2.1.8). L'invention décrit également de quelle façon il est possible d'utiliser une mutagenèse portant sur un site afin d'améliorer les capacités d'une enzyme à satisfaire les conditions industrielles dans le cadre desquelles elle est utilisée. Il est possible de mettre en oeuvre cette ingénierie des protéines pour améliorer l'activité thermique et la stabilité thermique de xylanases ainsi que pour élargir la gamme de leur pH.
Fenel Fred
Leisola Matti
Turunen Ossi
Carbozyme Oy
Genencor International Inc.
Mccarthy Tetrault Llp
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