C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
150/13, 195/46,
C12N 5/12 (2006.01) C12N 5/16 (2006.01) C12P 21/00 (2006.01) G01N 33/48 (2006.01)
Patent
CA 1312030
METHOD OF INCREASING PRODUCT EXPRESSION THROUGH SOLUTE STRESS Abstract of the Disclosure A method of determining the optimal level of product expression and cell growth of animal cell cul- ture is described. The method generally comprises culturing cells under conditions of solute stress, that is, under conditions whereby optimal cell growth is decreased yet levels of product expression are in- creased. In a preferred embodiment of the invention is described a method of increasing the yield of mono- clonal antibodies comprising culturing hybridoma cells in an environment of solute stress. One approach to the creation of such an environment is the addition of inorganic salts, organic polyols, or metabolic prod- ucts to the culture medium. One to three fold in- creases in antibody yield have been obtained by these methods.
581361
Howarth William
Inlow Duane
Maiorella Brian
Borden Ladner Gervais Llp
Novartis Vaccines And Diagnostics Inc.
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