Method to isolate dna

Details Method to isolate dna Method to isolate dna

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2476456

Presented herewith is a method for isolating DNA from a biological sample. The methods comprise the following sequential steps: separating the biological material comprising DNA from the remainder of the biological sample; contacting the separated biological sample comprising DNA of step (a) with a hypertonic, high salt reagent so as to form a suspension of said biological material comprising DNA; contacting the suspension of step (b) with a lysis reagent to form a lysate comprising DNA and non-DNA biological components released from the biological material; and separating the DNA from the non-DNA biological components in the lysate of step (c) to yield isolated DNA.

L'invention concerne un procédé amélioré d'isolement d'ADN à partir d'échantillons biologiques.

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