C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) A61B 10/00 (2006.01) A61K 35/52 (2006.01) G01N 33/483 (2006.01) G01N 33/50 (2006.01)
Patent
CA 2055494
Intact X and Y chromosome bearing sperm populations of rabbits and swine were separated according to DNA content using a flow cytometer/cell sorter. Sperm viability was maintained by special staining techniques and by sorting and collecting the sperm in nutrient media. The sorted sperm were surgically inseminated into the uteri of rabbits or swine. Of the offspring born from does inseminated with the sorted population of X-bearing sperm, 94 % were females. Of offspring born from does inseminated with sorted Y-bearing sperm from the same ejaculate, 81% were males.
Des populations de spermes de lapins et de porcs comportant des chromosomes X et Y intacts ont été séparées selon leur contenu ADN au moyen d'un cytomètre à circulation/trieur de cellules. La viabilité du sperme a été maintenue au moyen de techniques de coloration spéciales ainsi qu'en triant et en conservant le sperme dans un milieu nitritif. Le sperme trié a été inséminé chirurgicalement dans des utérus de lapins ou de porcs. Des progénitures nées de femelles inséminées de populations triées de sperme comportant des chromosomes X, 94 % étaient des femelles. Des progénitures nées de femelles inséminées de sperme trié comportant des chromosomes Y provenant du même éjaculat, 81 % étaient des mâles.
Gowling Lafleur Henderson Llp
United States Of America Represented By The Secretary U.s. Department Of Commerce (the)
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