Method to produce sterile male flowers and partenocarpic...

Details Method to produce sterile male flowers and partenocarpic... Method to produce sterile male flowers and partenocarpic...

C - Chemistry, Metallurgy

12

N

C12N 15/29 (2006.01) A01H 1/00 (2006.01) A01H 3/00 (2006.01) A01H 5/00 (2006.01) C07K 14/415 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2615249

Method to produce sterile male flowers and parthenocarpic fruits by genetic silencing, said method comprising the steps of: a) obtaining the codifying sequence of Pistillata (PI)-homologous genes from the target species; b) analyzing the expression of the sequence obtained in step (a) to test its expression according to the pattern described for Pistillata genes; c) analyzing the complementation of PI-gene mutant with the PI sequence obtained from the target species, to assess that the obtained sequence fulfills the function of a PI gene; d) making a genetic silencing construct that comprises a region of the codifying sequence of PI in a plant expression vector; e) incorporation of the constructed vector into Agrobacterium tumefaciens; f) transforming target plants with Agrobacterium tumefaciens modified with the silencing vector and selecting said transformed plants; g) checking the absence of Agrobacterium contamination and corroborating transgenic plants by transgene amplification. Genes VvPI from Vitis vinifera cv. Cabernet Sauvignon and LePI from Lycopersicon esculentum are also described; together with the use of these genes to produce sterile male flowers and seedless or parthenocarpic fruits. Furthermore, silencing vectors that comprise these sequences or a part thereof are disclosed.

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