Method using a nonlinear optical technique for detection of...

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G01N 21/63 (2006.01) G01N 21/75 (2006.01) G01N 21/84 (2006.01) G01N 33/48 (2006.01) G01N 33/543 (2006.01)

Patent

CA 2488532

A nonlinear optical technique, such as second or third harmonic or sum or difference frequency generation, is used to detect binding interactions, or the degree or extent of binding, that comprise a conformational change. In one aspect of the present invention, the nonlinear optical technique detects a conformational change in a probe due to target binding. In another aspect of the invention, the nonlinear optical technique screens candidate probes by detecting a conformational change due to a probe-target interaction. In another aspect of the invention, the nonlinear optical technique screens candidate modulators of a probe-target interaction by detecting a conformational change in the presence of the modulator.

L'invention concerne une technique optique non linéaire, telle que la production d'une deuxième ou troisième harmonique ou somme ou fréquence de différence, utilisée pour détecter des interactions de liaison ou le degré ou l'extension de la liaison qui comprend une modification conformationnelle. Selon un aspect de l'invention, la technique optique non linéaire détecte une modification non conformationnelle dans une sonde provoquée par une liaison cible. Selon un autre aspect de l'invention, la technique optique non linéaire permet de séparer des sondes candidates en détectant une modification conformationnelle provoquée par une interaction sonde-cible. Dans un autre mode de réalisation, la technique optique non linéaire permet de séparer des modulateurs de candidats d'une interaction sonde-cible au moyen d'une détection d'une modification conformationnelle en présence du modulateur.

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