Methods and agents for detecting parechovirus

C - Chemistry – Metallurgy – 07 – H

Patent

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Details

C07H 21/04 (2006.01) C07H 21/00 (2006.01) C12N 15/11 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2651123

The present disclosure provides methods that permit detection of all known species of Parechovirus, including Human parechovirus and Ljungan virus (LV). In particular examples the method includes amplifying at least a portion of the 5 TSTTR of parechovirus nucleic acid molecules obtained from a sample, and detecting the resulting amplicons, but does not require culturing of the virus. The present disclosure also provides methods for determining which particular species or serotype of parechovirus is present in a biological sample. Also provided are oligonucleotide primers and probes that can be used in the disclosed methods.

La présente invention concerne des procédés qui permettent la détection de toutes les espèces connues de Parechovirus, y compris le parechovirus humain et le virus de Ljungan (LV). Dans des exemples particuliers, le procédé consiste à amplifier au moins une partie de la 5 TSTTR de molécules d'acides nucléiques de parechovirus prélevées dans un échantillon et à détecter les amplicons résultants, sans que la culture du virus ne soit requise. La présente invention concerne également des procédés destinés à déterminer quelle espèce particulière ou quel sérotype particulier de parechovirus est présent dans un échantillon biologique. L'invention concerne également des amorces et des sondes d'oligonucléotides qui peuvent être utilisées dans les procédés décrits ici.

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