Methods and apparatus for analyzing polynucleotide sequences...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01) C07H 21/02 (2006.01) C07H 21/04 (2006.01) B01L 3/00 (2006.01)

Patent

CA 2440754

The invention provides methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension. Some applications of the invention utilize total internal reflection fluorescence microscopy to image polynucleotide molecules at single molecule resolution.

L'invention concerne des procédés et un appareil d'analyse de séquences de polynucléotide par extension de base asynchrone. Certaines applications de l'invention utilisent la microscopie de fluorescence par réflexion interne totale afin d'imager les molécules de polynucléotide avec un niveau de résolution d'une seule molécule.

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