Methods and apparatus for dna sequencing

Details Methods and apparatus for dna sequencing Methods and apparatus for dna sequencing

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) B01J 19/00 (2006.01) B01L 3/00 (2006.01) C07H 21/00 (2006.01) C07H 21/04 (2006.01) C12N 15/10 (2006.01) G01N 21/64 (2006.01) G01N 27/447 (2006.01)

Patent

CA 2155186

The present invention provides a method and apparatus for automated DNA sequencing. The method of the invention includes the steps of: a) using a processive exonuclease to cleave from a single DNA strand the next available single nucleotide of the strand; b) transporting the single nucleotide away from the DNA strand; c) incorporating the single nucleotide in a fluorescence-enhancing matrix; d) irradiating the single nucleotide to cause it to fluoresce; e) detecting the fluorescence; f) identifying the single nucleotide by its fluorescence; and g) repeating steps a) to f) indefinitely (e.g., until the DNA strand is fully cleaved or until a desired length of the DNA is sequenced). The nucleotides are advantageously detected by irradiating the nucleotides with a laser to stimulate their natural fluorescence.

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