Methods and apparatus for dna sequencing and dna identification

Details Methods and apparatus for dna sequencing and dna identification Methods and apparatus for dna sequencing and dna identification

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) B01J 19/00 (2006.01)

Patent

CA 2206815

Sequencing by Hybridization (SBH) methods and apparatus employing subdivided filters for discrete multiple probe analysis of multiple samples may be used for DNA identification and for DNA sequencing. Partitioned filters are prepared. Samples are affixed to sections of partitioned filters and each sector is probed with a single probe or a multiplexed probe for hybridization scoring. Hybridization data is analyzed for probe complementarity, partial sequencing by SBH or complete sequencing by SBH.

Un appareil et des procédés de séquençage par hybridation employant des filtres subdivisés pour l'analyse par des sondes multiples distinctes d'échantillons multiples, peuvent être utilisés pour l'identification et le séquençage d'ADN. On prépare des filtres cloisonnés et on fixe des échantillons sur des parties de ces filtres cloisonnés qui sont sondées ensuite chacune par une sonde unique ou multiplexée pour donner un résultat d'hybridation. Les données d'hybridation sont analysées pour déterminer une complémentarité des sondes, ou un séquençage, partiel ou complet, par hybridation.

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