C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C07H 21/02 (2006.01) C07H 21/04 (2006.01) C12P 19/34 (2006.01) G01N 15/06 (2006.01) G01N 33/566 (2006.01)
Patent
CA 2350756
The present invention provides substrates and apparatuses for efficient, rapid and specific capture, and optimal recovery, of nucleic acids, as well as methods of their use. The substrate is porous in nature and has a capture polynucleotide capable of hybridizing to a target nucleic acid immobilized thereon. Upon flowing a sample containing or suspected of containing the target nucleic acid through the porous substrate, the target nucleic acid is rapidly captured. Following capture, the target nucleic acid can be efficiently recovered for subsequent use.
L'invention concerne des substrats et des appareils permettant de capturer des acides nucléiques de manière efficace, rapide et spécifique, et de les récupérer de manière optimale. L'invention concerne également les procédés d'utilisation desdits acides nucléiques. Le substrat est poreux par nature, et possède un polynucléotide de capture capable de s'hybrider avec un acide nucléique cible immobilisé sur ledit substrat. Lors de l'écoulement d'un échantillon contenant ou suspecté de contenir un acide nucléique cible qui traverse le substrat poreux, ledit acide nucléique est rapidement capturé. Suite à cette capture, l'acide nucléique cible peut être efficacement récupéré pour une utilisation ultérieure.
Chen Jer-Kang
Chiesa Claudia
Fry George A.
Furniss Vergine C.
Lambert Stephen M.
Applera Corporation
Osler Hoskin & Harcourt Llp
Perkin-Elmer Corporation The
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