Methods and apparatus for immunoploidy analysis

Details Methods and apparatus for immunoploidy analysis Methods and apparatus for immunoploidy analysis

G - Physics

01

N

167/37, 73/55, 1

G01N 33/74 (2006.01) C12Q 1/68 (2006.01) G01N 15/14 (2006.01) G01N 33/569 (2006.01) G01N 33/574 (2006.01) G01N 33/68 (2006.01)

Patent

CA 1312276

ABSTRACT OF THE DISCLOSURE A method and apparatus are used to measure selected features or parameters of cells by optical identification of marked cells. Preferably, an optical enhancement factor, such as a chromogen, is bound to a specific protein in the cytoplasm of a cell in order to type a cell. A monoclonal antibody specific to the cytoplasmic protein binds to the protein site and is magnified by an enzyme development technique. The DNA of the cell nucleus may also be stained. An image analysis may be made with two separate images with the stained DNA areas visible in one image and the optically enhanced cytoplasm areas visible in the second image. These images may also be combined. The cells are classified based on their morphological and/or optical markings.

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