Methods and apparatus for improved luminescence assays using...

Details Methods and apparatus for improved luminescence assays using... Methods and apparatus for improved luminescence assays using...

G - Physics

01

N

G01N 33/553 (2006.01) C12Q 1/68 (2006.01) C12Q 1/70 (2006.01) G01N 21/76 (2006.01) G01N 33/537 (2006.01) G01N 33/543 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2112675

What is described are methods and apparatus for performing a binding assay for an analyte of interest present in a sample. The methods include the steps of: forming a composition containing the sample, an assay-performance-substance which contains a component linked to a label compound capable of chemiluminescing when triggered, and a plurality of particles capable of specifically binding with the analyte and/or the assay-performance-substance; incubating the composition to form a complex which includes a panicle labeled component; collecting the complex in a collection zone; introducing into the collection zone a trigger capable of triggering the label such that the label luminesces; and measuring the emitted luminescence to measure the presence of the analyte of interest in the sample.

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