Methods and apparatus for low resistance electrophoresis of...

G - Physics – 01 – N

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G01N 27/447 (2006.01) G01N 27/453 (2006.01)

Patent

CA 2489725

Methods and apparatus are presented that facilitate electrophoresis of prior- cast, hydratable separation media, usefully immbobilized pH gradient (IPG) strips. The method exploits the swelling of prior-cast, hydratable separation media upon rehydration to help lodge the media in an enclosing member (10) that permits spaced electrical communication with the enclosed separation media. The electrical communication permits a voltage gradient to be established in the enclosed separation medium sufficient to effect separation of analytes therein. Cassettes (100), buffer cores (126), electrophoresis systems and kits are presented for effecting the methods of the invention.

La présente invention concerne des procédés et un appareil qui facilitent l'électrophorèse de milieux de séparation hydratables de pré-moulage, des bandes à gradient de pH immobilisé. Le procédé exploite le pouvoir de gonflement des milieux de séparation hydratables de pré-moulage après réhydratation, afin de maintenir les milieux logés dans un élément enveloppant qui permet une communication électrique espacée avec les milieux de séparation enveloppés. Cette communication électrique permet l'établissement d'un gradient de tension dans le milieu de séparation enveloppé suffisant pour induire une séparation des matières à analyser qui s'y trouvent. La présente invention concerne également des cassettes, des noyaux tampons, des systèmes et des trousses d'électrophorèse permettant de mettre en oeuvre les procédés selon cette invention.

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