Methods and apparatus for reducing protein content in sperm...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/076 (2010.01) A01N 1/02 (2006.01) C12M 1/34 (2006.01) C12M 3/00 (2006.01)

Patent

CA 2619951

The inventive technology relates to methods and apparatus for reducing protein content in sperm cell extenders and may include one or more of the following features: techniques for reducing protein content in a sperm cell extender; techniques for reducing protein content in a cryoprotectant-containing B fraction of a sperm cell extender; techniques for preparing sperm cell extenders that do not require clarification; techniques for preparing low density gradient sperm cell extenders suitable for centrifugation; techniques for reducing protein content between individual steps in preparing a sperm cell extender, and techniques for establishing novel values of reduced protein content in sperm cell extenders.

L'invention concerne des procédés et un appareil permettant de réduire la teneur en protéines dans des dilueurs de cellules spermatiques, qui consistent notamment en des techniques de réduction de la teneur en protéines dans un dilueur de cellule spermatique; des techniques de réduction de la teneur en protéines dans une fraction B contenant un cryoptrotecteur d'un dilueur de cellule spermatique; des techniques de préparation de dilueurs de cellules spermatiques à faible gradient de densité appropriés pour la centrifugation; des techniques de réduction de la teneur en protéines entre des étapes individuelles de préparation d'un dilueur de cellules spermatiques, et des techniques permettant d'établir de nouvelles valeurs d'une teneur réduite en protéines dans des dilueurs de cellules spermatiques.

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