Methods and apparatus for template capture and normalization...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) B01L 7/00 (2006.01)

Patent

CA 2380794

Methods for preparing nanoscale reactions using nucleic acids are presented. Nucleic acids are captured saturably, yet reversibly, on the internal surface of the reaction chamber, typically a capillary. Excess nucleic acid is removed and the reaction is performed directly within the capillary. Alternatively, the saturably bound nucleic acid is eluted, dispensing a metered amount of nucleic acid for subsequent reaction in a separate chamber. Devices for effecting the methods of the invention and a system designed advantageously to utilize the methods for high throughput nucleic acid sequencing reactions are also provided.

L'invention concerne des réactions à nanoéchelle au moyen d'acides nucléiques. Selon l'invention, les acides nucléiques sont captés de façon saturable mais réversible à la surface interne de la chambre de réaction, généralement d'un capillaire. L'acide nucléique en excès est évacué, et la réaction est effectuée directement à l'intérieur du capillaire. En variante, on procède à l'élution de l'acide nucléique lié à saturation et l'on distribue une quantité dosée d'acide nucléique pour procéder ensuite à une réaction dans une chambre séparée. L'invention concerne aussi des dispositifs de mise en oeuvre des procédés de l'invention et un système avantageux destiné à utiliser les procédés de l'invention pour des réactions haut débit de séquençage d'acides nucléiques.

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